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You are here:Home » Molecular Biology » Growth Factors-SCF » Stem Cell Factor, Recombinant, Rat (SCF, c-Kit Ligand, KL, Steel Factor, MGF)

Stem Cell Factor, Recombinant, Rat (SCF, c-Kit Ligand, KL, Steel Factor, MGF)

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Specifications

Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells.
Catalog #142982
SourceRecombinant protein corresponding to 165aa from rat SCF expressed in E.coli.
Molecular Weight~18.4kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by its ability to induce proliferation of TF-1 cells and is typically <10ng/ml.
AA SequenceMQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18.4kD
SourceE.coli
Purity~98% (Reducing, and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder. No additives. Reconstitute with sterile 10mM acetic acid.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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