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You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor Alpha, Recombinant, Human, Animal Free (TNF-alpha, AF, TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF)

Tumor Necrosis Factor Alpha, Recombinant, Human, Animal Free (TNF-alpha, AF, TNFSF2,
Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF)

Pricing

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Specifications

Tumor Necrosis Factor alpha (TNFa) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNFa activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNFR2, which is expressed mainly on immune cells. TNFa can have many functions including, to stimulate of phagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever.
Catalog #142995
SourceRecombinant protein corresponding to158aa from human TNF-a expressed in E.coli.
Molecular Weight~17.5kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D and is typically <0.05ng/ml.
AA SequenceMVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.5kD
SourceE.coli
Purity~96% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 10mM Na2PO4, pH 8.0. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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