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You are here:Home » Molecular Biology » MB-Obesity Proteins » Visfatin, Recombinant, Human (PBEF)

Visfatin, Recombinant, Human (PBEF)

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Specifications

Visfatin is an adipokine produced by visceral adipose tissue. Other adipokines include leptin, adiponectin, resistin, chemerin, omentin and vaspin. Visfatin has been shown to act as a pro-inflammatory factor for PBMCs and may mimic the effects of insulin on adipocytes, monocytes and hepatocytes.
Catalog #143000
SourceRecombinant protein corresponding to 466aa from human Visfatin expressed in E.coli.
Molecular Weight~52.6kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100ng/ml.
AA SequenceMPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~52.6kD
SourceE.coli
Purity~90% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder. No additives. Reconstitute with 20mM hydrochloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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