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143207 Mouse Anti-Keratin 7, CT (K7, CK7, SCL, K2C7, MGC3625, MGC129731)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
13k80
Grade
Affinity Purified
Applications
IC WB
Crossreactivity
Hu
Gene #
KRT7
Shipping Temp
Blue Ice
Storage Temp
-20°C
(K7, CK7, SCL, K2C7, MGC3625, MGC129731)

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia ining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunocytochemistry: 1:150 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant human Keratin 7 protein fragments expressed in E. coli.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.2% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of Keratin 7. Does not cross-react with related proteins. Species Crossreactivity: human
Conjugates
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