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You are here:Home » Molecular Biology » Growth Factors-Other » Amphiregulin, Recombinant, Human (AR, AREG, Colorectum cell-derived growth factor (CRDGF))

Amphiregulin, Recombinant, Human (AR, AREG, Colorectum cell-derived growth factor (CRDGF))

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Specifications

Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts.Amphiregulin also inhibits the growth of certain carcinoma cell lines.Synthesized as a transmembrane protein, Amphiregulinrsquos extracellular domain is proteolytically processed to release the mature protein.There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity.Recombinant human Amphiregulin is a 11.3kD glycoprotein consisting of 98 amino acid residues.
Catalog #143274
Biological ActivityDetermined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10ng/ml.
AA SequenceSVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity95% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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