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You are here:Home » Molecular Biology » MB-Growth Factors-CTGF » CTGFL/WISP-2, Recombinant, Human (Connective Tissue Growth Factor-Like protein, CCN5, CT-58)

CTGFL/WISP-2, Recombinant, Human (Connective Tissue Growth Factor-Like protein, CCN5,
CT-58)

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Specifications

CTGFL/WISP-2 is a 28.6kD protein that belongs to the CCN family of cysteine rich regulatory proteins.Members of this family stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types.The protein is expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.In addition to promoting adhesion of osteoblasts, CTGFL/WISP-2 inhibits osteocalcin production, as well as binding of fibrinogen to integrin receptors. Recombinant human CTGFL/WISP-2 is a 24.3kD protein of 228 amino acid residues.
Catalog #143304
Biological ActivityThe ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expectd ED50 is between 10-20ng/ml in the presence of 15ng/ml of human IGF-II.
Amino Acid SequenceMQLCPTPCTCPWPPPRCPLGVPLVLDGCGCCRVCARRLGEPCDQLHVCDASQGLVCQPGAGPGGRGALCLLAEDDSSCEVNGRLYREGETFQPHCSIRCRCEDGGFTCVPLCSEDVRLPSWDCPHPRRVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQFSGLVSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVSNQNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity95% by SDS-PAGE gel and HPLC analyses. Endotoxin:
0.1ng/ug ( LAL)
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not raise to neutral pH and do not vortex.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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