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You are here:Home » Molecular Biology » Growth Factors-FGF » FGF-18, Recombinant, Human (Fibroblast Growth Factor-18, zFGF5, FGFI)

FGF-18, Recombinant, Human (Fibroblast Growth Factor-18, zFGF5, FGFI)

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Specifications

FGF-18 is a heparin binding growth factor that belongs to the FGF family.Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.FGF-18 is an essential regulator of long bone and calvarial development.FGF-18 signals through FGFR 1c, 2c, 3c, and 4.Recombinant E.coli derived human FGF-18 is a 20.1kD protein consisting of 173 amino acid residues, resulting from c-terminal truncation of the full length protein.
Catalog #143321
AA SequenceAEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPEL QKPFKYTTVT KRSR
Verified by UV Spectroscopy and/or SDS-PAGE gel.
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Biological ActivityASSAY #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ~0.5ng/ml, corresponding to a specific activity of ~2x10e6 units/mg.
ASSAY #2: The ED50 as determined by cell proliferation assay using balb/c 3T3 cells. The expected ED50 is <10.0ng/ml,
corresponding to a specific activity of 1x10e5 units/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS NumberN/A
SourceE. coli
Purity95% by SDS-PAGE gel and HPLC analyses. Endotoxin:
0.1ng/ug of protein (<1EU/ug).
FormSupplied as a lyophilized powder from10mM Tris pH8.0, 20mM sodium chloride. Reconstitute in sterile dH20 to a concentration of 0.1-1mg/ml. Do not vortex.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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