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You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » FGF-basic, Recombinant, Mouse (Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin)

FGF-basic, Recombinant, Mouse (Fibroblast Growth Factor-basic, FGF-2, HBGF-2,
Prostatropin)

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Specifications

FGF-basic is one of 23 known members of the FGF family.Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells.FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.Recombinant murine FGF-basic is a 16.3kD protein consisting of 145 amino acid residues.
Catalog #143326
Biological ActivityAssay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ~0.1ng/ml corresponding to a specific activity of ~1x10e7 units/mg.
Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ~1.0ng/ml, corresponding to a specific activity of ~1x10e6 units/mg.
AA SequencePALPEDGGAA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
CrossreactivityRabbit
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity95% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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