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You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » FGF-basic (146 a.a.), Recombinant, Human (Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin)

FGF-basic (146 a.a.), Recombinant, Human (Fibroblast Growth Factor-basic, FGF-2, HBGF-2,
Prostatropin)

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Specifications

FGF-basic is one of 23 knownmembers of the FGF family. Proteins of this family play a central role duringprenatal development and postnatal growth and regeneration of a variety oftissues, by promoting cellular proliferation and differentiation. FGF-basic isa non-glycosylated heparin binding growth factor that is expressed in thebrain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes,epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c,2c, 3c and 4. Recombinant Human FGF-basic (146aa) is a 16.4kD proteinconsisting of 146 amino acid residues.
Catalog #143328
Biological ActivityThe ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ~0.05ng/ml, corresponding to a specific activity of ~2x10e7 units/mg.
AA SequencePALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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