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You are here:Home » Growth Factors, Cytokines » Growth Factors-G-CSF » G-CSF, Recombinant, Rat (Granulocyte, Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSFbeta, pluripoietin)

G-CSF, Recombinant, Rat (Granulocyte, Colony-Stimulating Factor, CSF-3, MGI-1G,
GM-CSFbeta, pluripoietin)

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Specifications

G-CSF is a hematopoieticgrowth factor that stimulates the development of committed progenitor cells toneutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specificstimulation by a variety of cells including macrophages, fibroblasts,endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery afterbone marrow transplantation. Recombinant rat G-CSF is a 21.6kD proteinconsisting of 196 amino acid residues.
Catalog #143339
Biological ActivityDetermined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED50 is ~0.05ng/ml, corresponding to a specific activity of ~2x10e7 units/mg.
AA SequenceMKKIPLLTVS SLPPSLPLPR SFLLKSLEQV RKIQARNTEL LEQLCATYKL CHPEELVLFG HSLGIPKASL SSCSSQALQQ TKCLSQLHSG LFLYQGLLQA LAGISSELAP TLDMLHLDVD NFATTIWQQM ESLGVAPTVQ PTQSTMPIFT SAFQRRAGGV LVTSYLQSFL ETAHHALHHL PRPAQKHFPE SLFISI
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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