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You are here:Home » Growth Factors, Cytokines » Growth Factors-TGF » Gremlin-1, Recombinant, Human (CKTSF1B1, DAND2, DRM, IHG-2)

Gremlin-1, Recombinant, Human (CKTSF1B1, DAND2, DRM, IHG-2)

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Specifications

Gremlin-1 (isoform-1) belongsto a group of diffusible proteins which bind to ligands of the TGF-beta family andregulate their activity by inhibiting their access to signaling receptors. Theinterplay between TGF-beta ligands and their natural antagonists has majorbiological significance during development processes, in which cellularresponse can vary considerably depending upon the local concentration of thesignaling molecule. Gremlin is highly expressed in the small intestine, fetalbrain, and colon and lower expression in brain, prostate, pancreas and skeletal muscle. Gremlin-1 regulates multiple functions in early development byspecifically binding to and inhibiting the function of BMP-2, -4, and -7. It also plays a role in carcinogenesis and kidney branching morphogenesis. RecombinantGremlin-1 is a 18.3kDa proteincontaining 160 amino acid residues.
Catalog #143352
Biological ActivityDetermined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11ug/ml.
AA SequenceKKKGSQGAIP PPDKAQHNDS EQTQSPQQPG SRNRGRGQGR GTAMPGEEVL ESSQEALHVT ERKYLKRDWC KTQPLKQTIH EEGCNSRTII NRFCYGQCNS FYIPRHIRKE EGSFQSCSFC KPKKFTTMMV TLNCPELQPP TKKKRVTRVK QCRCISIDLD
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceCHO cells
Purity90% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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