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You are here:Home » Molecular Biology » Growth Factors-HGF » HGF, Recombinant, Mouse (Scatter Factor (SF), Hepatopoietin (HPTA))

HGF, Recombinant, Mouse (Scatter Factor (SF), Hepatopoietin (HPTA))

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Specifications

HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types.HGF signals through a transmembrane tyrosine kinase receptor known as MET.Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival.HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries.Human and murine HGF are cross-reactive.Murine HGF is expressed as a linear 728 amino acid polypeptide precursor glycoprotein.Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer.The alpha-chain consists of 463 amino acid residues and four kringle domains.The beta-chain consists of 233 amino acid residues.
Catalog #143362
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20ng/ml.
AA Sequence<B>Alpha chain:</B> QKKRRNTLHE FKKSAKTTLT KEDPLLKIKT KKVNSADECA NRCIRNRGFT FTCKAFVFDK SRKRCYWYPF NSMSSGVKKG FGHEFDLYEN KDYIRNCIIG KGGSYKGTVS ITKSGIKCQP WNSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG PMDHTESGKT CQRWDQQTPH RHKFLPERYP DKGFDDNYCR NPDGKPRPWC YTLDPDTPWE YCAIKTCAHS AVNETDVPME TTECIQGQGE GYRGTSNTIW NGIPCQRWDS QYPHKHDITP ENFKCKDLRE NYCRNPDGAE SPWCFTTDPN IRVGYCSQIP KCDVSSGQDC YRGNGKNYMG NLSKTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNKNYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR</br><B>Beta chain:</B> VVNGIPTQTT VGWMVSLKYR NKHICGGSLI KESWVLTARQ CFPARNKDLK DYEAWLGIHD VHERGEEKRK QILNISQLVY GPEGSDLVLL KLARPAILDN FVSTIDLPSY GCTIPEKTTC SIYGWGYTGL INADGLLRVA HLYIMGNEKC SQHHQGKVTL NESELCAGAE KIGSGPCEGD YGGPLICEQH KMRMVLGVIV PGRGCAIPNR PGIFVRVAYY AKWIHKVILT YKL
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
Source(BTI-Tn-5B1-4) Hi-5 Insect cells
Purity95% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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