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You are here:Home » Molecular Biology » Growth Factors-IGF » IGF-BP1, Recombinant, Human (Insulin-like Growth Factor-Binding Protein 1, IBP-1, Placenta Protein 12 (PP12))

IGF-BP1, Recombinant, Human (Insulin-like Growth Factor-Binding Protein 1, IBP-1, Placenta
Protein 12 (PP12))

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Specifications

IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids.Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II.IGF-BP1 is a 25.4kD cysteine-rich secreted protein expressed in liver, deciduas, and kidneys and is the most abundant IGF-BP in amniotic fluid.Levels of IGF-BP1 in serum are lowest after food.IGF-BP1 binds to both IGF-I and IGF-II with equal affinity.Phosphorylated IGF-BP1 hinders IGF actions, where as nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a 25.4kD protein consisting of 235 amino acid residues (Isoform A).
Catalog #143372
Biological ActivityThe ED50 was determined by its ability to inhibit IGF-I induced proliferation of MCF-7 is ~0.5ug/ml in the presence of 6ng/ml of human IGF-I.
AA SequenceMAPWQCAPCSAEKLALCPPVSASCSEVTRSAGCGCCPMCALPLGAACGVATARCARGLSCRALPGEQQPLHALTRGQGACVQESDASAPHAAEAGSPESPESTEITEEELLDNFHLMAPSEEDHSILWDAISTYDGSKALHVTNIKKWKEPCRIELYRVVESLAKAQETSGEEISKFYLPNCNKNGFYHSRQCETSMDGEAGLCWCVYPWNGKRIPGSPEIRGDPNCQIYFNVQN
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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