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You are here:Home » Molecular Biology » Growth Factors-IGF » IGF-BP5, Recombinant, Human (Insulin-like Growth Factor-Binding Protein5)

IGF-BP5, Recombinant, Human (Insulin-like Growth Factor-Binding Protein5)

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Specifications

IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids.Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II.IGF-BP5 is a 28.6kD cysteine-rich secreted protein produced by vascular smooth muscle cells.It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts.Data shows that IGFBP-5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells.IGFBP-5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.Recombinant human IGF-BP5 is a 28.6kD protein consisting of 253 amino acid residues.
Catalog #143376
Biological ActivityThe ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is ~0.3ug/ml in the presence of 15ng/ml of human IGF-II.
AA SequenceMLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVS
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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