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You are here:Home » Molecular Biology » MB-Growth Factors-Interleukin » IL-21, Recombinant, Human

IL-21, Recombinant, Human


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IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation.Its action generally enhances antigen-specific responses of immune cells.The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naiumlve B-cells and stimulated B-cells in the absence of T-cell signaling.Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells.IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (gammac) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15.The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.Recombinant human IL-21 is a 15.4kD protein consisting of 132 amino acid residues.
Catalog #143397
Biological ActivityAssay #1: Determined by its ability to proliferate activated B cells.
Assay #2:Determined by its ability to induce human CD40L-activated native B cells to undergo Ig isotype switching to IgG, using an IL-21 concentration of 50ng/ml. Maximal activity was achieved after approximately five cell divisions.
Avery, D.T. et. al. (2008) J. Immunol<em>. 181, 1767-1779.
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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