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You are here:Home » Growth Factors, Cytokines » Growth Factors-MIF » MIF, Recombinant, Human (Macrophage Migration, Inhibitory Factor, GLIF, MMIF, GIF, Glycosylation-inhibiting factor)

MIF, Recombinant, Human (Macrophage Migration, Inhibitory Factor, GLIF, MMIF, GIF,
Glycosylation-inhibiting factor)

Pricing

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Specifications

Macrophage MigrationInhibitory Factor (MIF) is a small secreted protein that can act as apleiotropic pro-inflammatory cytokine as well as an enzyme. MIFpro-inflammatory activity can be initiated by signaling through CD74 and CD44,resulting in the secretion of TNF-alpha, IL-1, IL-6, IL-8, and various MMPs. Theenzymatic activity of MIF is characterized by its ability to act as atautomerase, capable of catalyzing the keto to enol isomerization ofketo-phenylpyruvate and L-dopachrome. It appears as though MIF catalyticactivity is dependent upon a trimeric configuration and a free N-terminalproline residue. Insect cell derived recombinant MIF is a 15kD proteincontaining 124 amino acid residues, including an N-terminal His-tag.
Catalog #143435
Biological ActivityDetermined by its ability to inhibit monocyte migration.
AA SequenceHHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceHi-5 Insect cells
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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