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You are here:Home » Molecular Biology » MB-Obesity Proteins » Nesfatin-1, Recombinant, Human (NES1)

Nesfatin-1, Recombinant, Human (NES1)

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Specifications

Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2). Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets beta-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Recombinant human Nesfatin-1 is a 9.7kD protein containing 82aa residues.
Catalog #143446
Biological ActivityDetermined by an in vivo assay using healthy wild type male mice (C57BL/6J). Mice were treated via intraperitoneal injection once at a dose of 4ug Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.
AA SequenceVPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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