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You are here:Home » Molecular Biology » Growth Factors-Neurotrophins » NT-3, Recombinant, Human (Neurotrophin-3, Neurotrophic factor, Nerve growth factor-2, NGF-2, HGNF)

NT-3, Recombinant, Human (Neurotrophin-3, Neurotrophic factor, Nerve growth factor-2,
NGF-2, HGNF)

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Specifications

NT-3 is a neurotrophic factor structurally related to beta-NGF, BDNF, and NT-4.These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures.NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors.NT-3 promotes the growth and survival of nerve and glial cells.The amino acid sequences of human, murine and rat NT-3 are identical.Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (240 total amino acid residues).Human and Mouse NT-3 sequences are identical.
Catalog #143454
Biological ActivityThe ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50ng/ml.
AA SequenceMYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT
CrossreactivityChicken, Bovine, Canine, Frog, Hamster, Mouse, Pig, Rat
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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