Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-Oncostatin M » Oncostatin-M, Recombinant, Human, 209aa (OSM)

Oncostatin-M, Recombinant, Human, 209aa (OSM)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activate T cells, monocytes and Kaposirsquos sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposirsquos sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. The human OSM gene encodes for a 252aa polypeptide, containing 25aa signal sequence for secretion and a 227 precursor protein. Proteolytic processing of this precursor removes an 18aa C-terminal peptide and generates the mature OSM form. Recombinant human Oncostatin M is a 23.6kD protein, containing 209aa residues.
Catalog #143455
Biological ActivityThe ED50as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ~2ng/ml, corresponding to a specific activity of ~5x10e5 units/mg.
AA SequenceAAIGSCSKE YRVLLGQLQK QTDLMQDTSR LLDPYIRIQG LDVPKLREHC RERPGAFPSE ETLRGLGRRG FLQTLNATLG CVLHRLADLE QRLPKAQDLE RSGLNIEDLE KLQMARPNIL GLRNNIYCMA QLLDNSDTAE PTKAGRGASQ PPTPTPASDA FQRKLEGCRF LHGYHRFMHS VGRVFSKWGE SPNRSRRHSP HQALRKGVRR
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links