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You are here:Home » Growth Factors, Cytokines » Growth Factors-SCF » SCF, Recombinant, Rat (Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor)

SCF, Recombinant, Rat (Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF),
Steel Factor)

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Specifications

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor.SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages.Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells.Recombinant rat SCF is an 18.4kD polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
Catalog #143494
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of the human TF-1 cells. The expected ED50 for this effect is 20-40ng/ml.
AA SequenceMQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
CrossreactivityHuman, Mouse
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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