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You are here:Home » Molecular Biology » Growth Factors-VEGF » VEGF121, Recombinant, Human (Vascular Endothelial Growth Factor121, VPF)

VEGF121, Recombinant, Human (Vascular Endothelial Growth Factor121, VPF)

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Specifications

VEGF is a potent growth and angiogenic cytokine.It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability.Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis.VEGF signals through three receptors fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.Due to its increased acidity, VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.Recombinant human VEGF121 is a 28.4kD disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.
Catalog #143537
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4ng/ml.
AA SequenceAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENCDKPRR
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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