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You are here:Home » Growth Factors, Cytokines » Growth Factors-VEGF » VEGF-B, Recombinant, Human (Vascular Endothelial Growth Factor-B, VEGF-related factor, VRF)

VEGF-B, Recombinant, Human (Vascular Endothelial Growth Factor-B, VEGF-related factor,
VRF)

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Specifications

VEGF-B, a member of the VEGF family, is a potent growth and angiogenic cytokine.It promotes DNA synthesis in endothelial cells, helps regulate angiogenesis and vascular permeability, and inhibits apoptosis in certain smooth muscle cells and neurons.VEGF-B is expressed in all tissues except the liver.It forms cell surfaced-associated disulfide linked homodimers and can form heterodimers with VEGF-A.There are two known isoforms, formed by alternative splicing, which have been designated VEGF-B167 and VEGF-B186.Both forms have identical amino-terminal sequences encoding a cysteine knotrdquo like structural motif, but differ in their carboxyl-terminal domains.Both VEGF-B isoforms signal only through the VEGFR1 receptor.Recombinant human VEGF-B is a 38.0kD disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.
Catalog #143538
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of human VEGF165. The expected ED50 for this effect is 1.0-2.0ug/ml.
AA SequencePVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDSPRPLCPRCTQHHQRPDPRTCRCRCRRRSFLRCQGRGLELNPDTCRCRKLRR
Quality ControlVerified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin<0.1ng/ug of protein (<1EU/ug).
Protein ContentVerified by UV Spectroscopy and/or SDS-PAGE gel.
Storage and StabilityStore lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.
SourceE. coli
Purity98% by SDS-PAGE gel and HPLC analyses.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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