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You are here:Home » Antibodies » Abs to Ligands » Anti -4-1BB Ligand (TNFSF9, CD137, CDw137, ILA, MGC2172, T-cell, 4-1BB Homolog, T-cell Antigen ILA, Tumor Necrosis Factor Receptor Superfamily Member 9 Precursor)

Anti -4-1BB Ligand (TNFSF9, CD137, CDw137, ILA, MGC2172, T-cell, 4-1BB Homolog, T-cell
Antigen ILA, Tumor Necrosis Factor Receptor Superfamily Member 9 Precursor)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
4-1BB, also known as CD137, is a type I transmembrane protein belonging to the TNF receptor superfamily (TNFRSF9). It is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector functions. On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.
Catalog #143547
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: To detect h4-1BBL by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.
Western Blot: To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant h4-1BBL is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry (Formalin fixed paraffin embedded): This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified human 4-1BB Ligand. Peprotech Cat No 310-11
SpecificityRecognizes human 4-1BB Ligand
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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