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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -APRIL (Tumor Necrosis Factor Ligand Superfamily Member 13, A Proliferation-inducing Ligand, TNFSF13, CD256, TNF- and APOL-related Leukocyte Expressed Ligand 2, TALL2, TALL-2, TNF-related Death Ligand 1, TRDL-1, UNQ383/PRO715, ZTNF2) (Biotin)

Anti -APRIL (Tumor Necrosis Factor Ligand Superfamily Member 13, A Proliferation-inducing
Ligand, TNFSF13, CD256, TNF- and APOL-related Leukocyte Expressed Ligand 2, TALL2, TALL-2,
TNF-related Death Ligand 1, TRDL-1, UNQ383/PRO715, ZTNF2) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
APRIL (a proliferation inducing ligand) is a member of the TNF ligand superfamily. It is synthesized as a 32kD type II transmembrane protein which is cleaved by furin in the Golgi to release a 17kD soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C-terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% aa sequence identity with mouse and rat APRIL.
Catalog #143550
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: To detect hAPRIL by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required.
ELISA (Direct): To detect hAPRIL by direct ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required.
Western Blot: To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4, conjugated to biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified human APRIL. Peprotech Cat No 310-10
SpecificityRecognizes human APRIL
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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