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You are here:Home » Antibodies » Abs to Growth Factors, CXCL » Anti -CXCL5 (C-X-C Motif Chemokine 5, ENA-78 (1-78), Epithelial-derived Neutrophil-activating Protein 78, Neutrophil-activating Peptide ENA-78, Small-inducible Cytokine B5, ENA78, SCYB5)

Anti -CXCL5 (C-X-C Motif Chemokine 5, ENA-78 (1-78), Epithelial-derived
Neutrophil-activating Protein 78, Neutrophil-activating Peptide ENA-78, Small-inducible
Cytokine B5, ENA78, SCYB5)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #143562
CXCL5 signals through binding to CXC receptor 2 (CXCR2) and occurs naturally in three isoforms ENA 5-78 (74aa), ENA 9-78 (70aa) and ENA 10-78 (69aa).
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: To detect hENA-78 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.
Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1-2ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified human ENA-78. Peprotech Cat No 300-22
SpecificityRecognizes human ENA-78
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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