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You are here:Home » Antibodies » Abs to Angiogenesis » Anti -Endostatin (Collagen alpha-1(XVIII) Chain, COL18A1) (Biotin)

Anti -Endostatin (Collagen alpha-1(XVIII) Chain, COL18A1) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Endostatin is a naturally occurring 20kD polypeptide derived from the C-terminal portion of type XVIII collagen. It functions as an anti-angiogenic cytokine that is expressed in various organs with the highest levels in liver, lung and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant human Endostatin is a 20.2kD protein consisting 184aa residues.
Catalog #143564
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: To detect hEndostatin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required.
ELISA (Direct): To detect hEndostatin by direct ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4ng/well of recombinant hEndostatin.
Western Blot: To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4, conjugated to biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified human Endostatin. Peprotech Cat No 150-01
SpecificityRecognizes human Endostatin
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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