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You are here:Home » Antibodies » Antibodies-Protease Inhibitors » Anti -Maspin (SERPINB5, Serpin B5, Peptidase Inhibitor 5, PI-5, PI5)

Anti -Maspin (SERPINB5, Serpin B5, Peptidase Inhibitor 5, PI-5, PI5)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Maspin, an abbreviated name for mammary-specific serpin is a tumor suppressor protein present in normal breast and prostatic epithelial cells but down-regulated in carcinomas of these cell types. The tumor suppressor action of maspin may be related to its ability to inhibit angiogenesis. Maspin mRNA has been found in various rat tissues but not in heart, lung, brain or kidney.
Catalog #143646
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant hMaspin.
Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8ng/ml-15.625ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified human Maspin. Peprotech Cat No 130-12
SpecificityRecognizes human Maspin
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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