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You are here:Home » Antibodies » Abs to Resistin » Anti -RETNLB (Resistin-like beta, Colon and Small Intestine-specific Cysteine-rich Protein, Colon Carcinoma-related Gene Protein, Cysteine-rich Secreted Protein A12-alpha-like 1, Cysteine-rich Secreted Protein FIZZ2, RELMbeta, CCRG, FIZZ2, HXCP2, RETNL2, UNQ408

Anti -RETNLB (Resistin-like beta, Colon and Small Intestine-specific Cysteine-rich
Protein, Colon Carcinoma-related Gene Protein, Cysteine-rich Secreted Protein
A12-alpha-like 1, Cysteine-rich Secreted Protein FIZZ2, RELMbeta, CCRG, FIZZ2, HXCP2,
RETNL2, UNQ408

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Human RELM beta (Resistin-like molecule beta/FIZZ2) is a new member to the family of adipocyte secreted proteins called adipocytokines. This family includes the RELM alpha, RELM beta and Resistin molecules. Interestingly, RELM beta and Resistin share similar characteristics such as an additional cysteine residue within the variable N-terminal region and are both homodimeric proteins. However, the RELM beta is expressed only in the gastrointestinal track; especially the colon, while the Resistin and RELM beta are secreted exclusively by adipocytes. Currently, the biological function of these proteins, as well as their molecular targets is largely unknown.
Catalog #143668
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: To detect Human RELMbeta; by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human RELMbeta as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant Human RELMbeta.
Western Blot: To detect Human RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human RELMbeta is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified human RELM . Peprotech Cat No 450-22
SpecificityRecognizes human RELM
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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