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143717 Angiostatin K1-3 BioAssay™ ELISA Kit, Human

Specifications
References
Brand
BioAssay™
Gene ID
5340
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 10 pg/ml
Detection Method
Colorimetric
Detection Range
156pg/ml-10,000pg/ml
Sample Matrix
cell culture supernates, serum and plasma
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Human Angiostatin K1-3 in cell culture supernates, serum and plasma.

Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED₅₀ =70 nM) relative to kringles 1-4 (ED₅₀ = 135 nM).
Assay Range
156pg/ml-10,000pg/ml
Sensitivity
< 10 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
P00747
Entrez Gene ID
5340
Kit Components
96-well plate pre-coated with anti-Angiostatin K1-3, Human Lyophilized recombinant Angiostatin K1-3, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
1. O’Relly MS, Holmgren L, Shin Y, Chen C, Rosenthal RA, Moses M et al. Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 1994; 79: 315–328|2. Cao Y, Ji RW, Davidson D, Schaller J, Marti D, Sohndel S et al. Kringle domains of human angiostatin. JBC 1996; 271: 29461–29467.|
USBio References
No references available
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