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143923 IL-33 BioAssay™ ELISA Kit, Mouse

Specifications
References
Brand
BioAssay™
Gene ID
77125
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 10 pg/ml
Detection Method
Colorimetric
Detection Range
31.2pg/ml-2000pg/ml
Sample Matrix
cell culture supernates, serum and plasma (heparin, EDTA)
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Mouse IL-33 in cell culture supernates, serum and plasma (heparin, EDTA).

Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
Assay Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
Q8BVZ5
Entrez Gene ID
77125
Kit Components
96-well plate pre-coated with anti-IL-33, Mouse Lyophilized recombinant IL-33, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
1. Bonilla, W. V., Frohlich, A., Senn, K., Kallert, S., Fernandez, M., Johnson, S., Kreutzfeldt, M., Hegazy, A. N., Schrick, C., Fallon, P. G., Klemenz, R., Nakae, S., Adler, H., Merkler, D., Lohning, M., Pinschewer, D. D. The alarmin interleukin-33 drives protective antiviral CD8+ T cell responses. Science 335: 984-989, 2012.|2. Schmitz, J., Owyang, A., Oldham, E., Song, Y., Murphy, E., McClanahan, T. K., Zurawski, G., Moshrefi, M., Qin, J., Li, X., Gorman, D. M., Bazan, J. F., Kastelein, R. A. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 23: 479-490, 2005.|
USBio References
No references available
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