sVEGFR1/sFLT1 BioAssay™ ELISA Kit, Human

Brand

BioAssay™

Gene ID

2321

Kit Type

Sandwich ELISA

Tests

Sample Volume

100ul

Sensitivity

<30 pg/ml

Detection Method

Colorimetric

Detection Range

156pg/ml-10, 000pg/ml

Sample Matrix

cell culture supernates, serum and plasma (EDTA)

EU Commodity Code

38220000

UN DOT Shipping

UN2796 PGII

Shipping Temp

Blue Ice

Storage Temp

4°C/-20°C

sVEGFR1 is also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is maps to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with a molecular mass of 100kD.

Intended Use

The sVEGFR1/sFLT1 BioAssay™ ELISA Kit, Human is a sandwich ELISA quantitative detection of human sVEGFR1/sFLT1 in cell culture supernates, serum and plasma (EDTA).

Detection Range

156-10,000pg/ml

Sensitivity

<30pg/ml

Specificity

Natural and recombinant human sVEGFR1/sFLT1

Crossreactivity

No detectable crossreactivity with other relevant proteins

Kit Components

144047A: Microtiter Strip, 1x96 wells 144047B: sVEGFR1/sFLT1 Standard, 2x10ng 144047C: Anti-human sVEGFR1/sFLT1 (Biotin), 1x100ul (1:100 dilution)

USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x100ul (1:100 dilution) 143697: Sample Diluent Buffer, 1x30ml 143698: Antibody Diluent Buffer, 1x12ml 143699: ABC Diluent Buffer, 1x12ml 143700: TMB Color Developing Agent, 1x10ml 143701: TMB Stop Solution, 2N H2SO4, 1x10ml 143702: Wash Buffer, 25X, 1x20ml

Storage and Stability

Store *144047B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Assay Summary

1. Add 100ul of samples and standards and incubate the plate at 37°C for 90 minutes or at RT for 2 hours. Do not wash. 2. Add 100ul biotinylated antibody and incubate the plate at 37°C for 60 minutes or at RT for 90 minutes. Wash plate 3 times with Working Wash Buffer. 3. Add 100ul of ABC working solution and incubate the plate at 37°C for 30 minutes or at RT for 40 minutes. Wash plate 5 times with Working Wash Buffer. 4. Add 90ul of TMB color developing agent and incubate the plate at 37°C in dark for 15-25 minutes or at RT for 30 minutes. 5. Add 100ul TMB Stop Solution and read.

References

1. Imbert, A.; Rosnet, O.; Marchetto, S.; Ollendorff, V.; Birnbaum, D.; Pebusque, M.-J. : Characterization of a yeast artificial chromosome from human chromosome band 13q12 containing the FLT1 and FLT3 receptor-type tyrosine kinase genes. Cytogenet. Cell Genet. 67: 175-177, 1994. 2. Niida, S.; Kondo, T.; Hiratsuka, S.; Hayashi, S.-I.; Amizuka, N.; Noda, T.; Ikeda, K.; Shibuya, M. : VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. Proc. Nat. Acad. Sci. 102: 14016-14021, 2005.

USBio References

No references available

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144047 sVEGFR1/sFLT1 BioAssay™ ELISA Kit, Human

Brand

Gene ID

Kit Type

Tests

Sample Volume

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Detection Range

Sample Matrix

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