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You are here:Home » Antibodies » Abs to Aquaporin » Anti -AQP2 (ADH water channel, Aquaporin-CD)

Anti -AQP2 (ADH water channel, Aquaporin-CD)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
AQP2(Aquaporin 2) also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus .The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide (NO). The NO donors sodium nitroprusside (SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin (VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.
Catalog #144189
UniProt NumberP41181
Gene IDAQP2
ApplicationsSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and HandlingStore at -20 C for one year. After reconstitution, store at 4 C for one month. Can also be aliquoted and stored frozen at -20 C for long term.
Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminal of human AQP2, identical to the related rat and mouse sequences.
SpecificityRecognizes human, mouse and rat AQP2. No crossreactivity with other proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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