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You are here:Home » Antibodies » Antibodies-Enzymes, Isomerase » Anti -ECI1 (3, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase)

Anti -ECI1 (3, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an
enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon
(position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the
metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of
enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray
crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold
composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA
Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid
degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so
by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or
trans-enoyl-CoA to 2-trans-enoyl-CoA.
Catalog #144472
UniProt NumberP42126
ApplicationsSuitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and HandlingStore at -20 C for one year. After reconstitution, store at 4 C for one month. Can also be aliquoted and stored frozen at -20 C for long term.
Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminal of human ECI1, different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.
SpecificityRecognizes human, mouse and rat ECI1. No crossreactivity with other proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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