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You are here:Home » Antibodies » Abs to Neurotrophins » Anti -GDNF (Astrocyte-derived trophic factor)

Anti -GDNF (Astrocyte-derived trophic factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands (GFL).The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization (FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene.
Catalog #144567
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.5ug/ml
Immunohistochemistry (Paraffin): 0.5-1ug/ml. Antigen retrieval by boiling paraffin sections in 10mM citrate buffer, pH 6.0 for 20mins is required for the staining of formalin/paraffin sections.
Immunocytochemistry: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 5% BSA, 0.2% Na2HPO4, 0.05% thimerosal, 0.9% sodium chloride, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa142-158, FRYCSGSCDAAETTYDK, a sequence in the middle region of human GDNF, different from the related mouse sequence by three amino acids and rat sequence by two amino acids.
SpecificityRecognizes human GDNF. Species crossreactivity: rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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