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You are here:Home » Antibodies » Abs to Thioredoxin » Anti -GLRX3 (PICOT, TXNL2, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, Thioredoxin-like protein 2)

Anti -GLRX3 (PICOT, TXNL2, PKC-interacting cousin of thioredoxin, PKC-theta-interacting
protein, Thioredoxin-like protein 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
GLRX3 (Glutaredoxin-3) is a protein that in humans is encoded by the GLRX3 gene. It is also known as Picot(Protein kinase C-interacting cousin of Thioredoxin). Hartz (2009)mapped the GLRX3 gene to chromosome 10q26.3 based on an alignment of the GLRX3 sequence with the genomic sequence (build 36.1), Using cotransfection and immunoprecipitation analysis with Jurkat cells, Witte et al. (2000) showed that PICOT interacted with PKC-theta and, more weakly, with PKC-zeta but not with PKC-alpha. Mutation analysis showed that the thioredoxin homology domain of PICOT was required for the interaction. Transfection of PICOT in Jurkat cells reduced basal JNK activity and significantly reduced PKC-theta-induced JNK activation. PICOT also inhibited the transcription factors AP1 and NF-kappa-B .Jeong et al. (2008)stated that Picot inhibits pressure overload-induced cardiac hypertrophy in rodents, concomitant with increased ventricular function and cardiomyocyte contractility. They showed that Picot colocalized with Mlp, which anchors calcineurin to the Z disc in the sarcomere and is critical for calcineurin-Nfat signaling. The C-terminal half of Picot inhibited cardiac hypertrophy, largely by disrupting the Mlp-calcineurin interaction and thereby negatively regulating calcineurin-Nfat signaling.
Catalog #144581
UniProt NumberO76003
Gene IDGLRX3
ApplicationsSuitable for use in Western Blot.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and HandlingStore at -20 C for one year. After reconstitution, store at 4 C for one month. Can also be aliquoted and stored frozen at -20 C for long term.
Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human GLRX3(158-176aa RCGFSKQMVEILHKHNIQF), identical to the related mouse sequence.
SpecificityRecognizes human, mouse and rat GLRX3. No crossreactivity with other proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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