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144739 Rabbit Anti-IL-3 (Hematopoietic growth factor, Mast cell growth factor)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P08700
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC IP N WB
Crossreactivity
Hu
Gene ID
IL3
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

UniProt Number
P08700
Gene ID
IL3
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry (Paraffin), Neutralization and Immunoprecipitation.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Handling
Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
E. coli-derived human IL-3 recombinant protein (Position: A20-F152).
Form
Supplied as a lyophilized powder. Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IL-3. No crossreactivity with other proteins.
References
1. Le Beau, M. M.; Epstein, N. D.; O'Brien, S. J.; Nienhuis, A. W.; Yang, Y.-C.; Clark, S. C.; Rowley, J. D. The interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987.|2. Clark-Lewis, I.; Aebersold, R.; Ziltener, H.; Schrader, J. W.; Hood, L. E.; Kent, S. B. H. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231: 134-139, 1986.|3. Fung, M. C.; Hapel, A. J.; Ymer, S.; Cohen, D. R.; Johnson, R. M.; Campbell, H. D.; Young, I. G. Molecular cloning of cDNA for murine interleukin-3. Nature 307: 233-237, 1984.|4. Ihle, J. N.; Silver, J.; Kozak, C. A. Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun. 138: 3051-3054, 1987.|5. Yang, Y.-C.; Kovacic, S.; Kriz, R.; Wolf, S.; Clark, S. C.; Wellems, T. E.; Nienhuis, A.; Epstein, N. The human genes for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71: 958-961, 1988.
USBio References
No references available
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