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You are here:Home » Antibodies » Abs to Sonic Hedgehog (SHH) » Anti -SHH (HHG-1)

Anti -SHH (HHG-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined; however, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
Catalog #145161
ApplicationsSuitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended DilutionWestern Blot: 0.1-0.5ug/ml
Immunohistochemistry (Paraffin): 0.5-1ug/ml. Antigen retrieval using heat is required. Boil paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes.
Optimal dilutions to be determined by the researcher.
Storage and HandlingLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to a sequence at the N-terminal of human SHH, identical to the related mouse and rat sequences.
SpecificityRecognizes SHH. Species Crossreactivity: rat. No crossreactivity with other proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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