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145314 Rabbit Anti-TRAM1 (TRAM, TRAMP, Translocating chain-associated membrane protein 1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q15629
Isotype
IgG
Grade
Affinity Purified
Applications
IC IHC WB
Crossreactivity
Hu Mo Rt
Gene ID
TRAM1
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

TRAM1 (Translocation-Associating Membrane Protein 1), By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al. (1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the TRAM gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

UniProt Number
Q15629
Gene ID
TRAM1
Applications
Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human TRAM1, identical to the related rat and mouse sequences.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human, mouse and rat TRAM1. No crossreactivity with other proteins.
References
1. Gorlich, D., Hartmann, E., Prehn, S., Rapoport, T. A. A protein of the endoplasmic reticulum involved early in polypeptide translocation. Nature 357: 47-52, 1992.
USBio References
No references available
Conjugates
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