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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -AMBP (Alpha 1-Microglobulin, A1M, AMBP, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI)

Anti -AMBP (Alpha 1-Microglobulin, A1M, AMBP, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC,
UTI)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Rat alpha 1­Microglobulin (alpha1­m/A1M; also protein HC) is a secreted 25­26kD glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. Alpha 1­Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. Alpha 1­Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Rat alpha 1­Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP­1, a 120­140kD adrenomedullin­binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N­terminal 183aa alpha 1­Microglobulin protein (aa20­202), and a C­terminal serine protease inhibitor termed bikunin (aa205­349). Alpha 1­Microglobulin possesses one lipocalin domain (aa41­186). Although cleavage of AMBP in the Golgi apparatus typically generates a 25kD alpha 1­Microglobulin and 28kD bikunin molecule, the 55­65kD AMBP precursor can also be released intact. In human, alpha 1­Microglobulin will undergo extracellular processing, generating an isoform that is missing the C­terminal four amino acids. There are four potential isoform variants. One utilizes an alternative start site at Met181, a second contains an Asn substitution for aa 112­228, a third possesses an 11aa substitution for aa1­141, and a fourth shows a 17aa substitution for aa201­349. Over aa20­202, rat alpha 1­Microglobulin shares 76% and 86% aa sequence identity with human and mouse alpha 1­Microglobulin, respectively.
Catalog #145388
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No771803
HostMouse
SourceRat
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
PurityPurified by Protein A or G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa20-202 from rat alpha 1 Microglobulin, expressed in E. coli (Accession# Q64240).
SpecificityRecognizes rat alpha 1­Microglobulin in ELISAs.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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