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You are here:Home » Antibodies » Antibodies-Cyclophilin » Anti -Cyclophilin A (CYPA, CYPH, PPIA, Rotamase, Peptidyl-prolyl Isomerase A)

Anti -Cyclophilin A (CYPA, CYPH, PPIA, Rotamase, Peptidyl-prolyl Isomerase A)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IC
Cyclophilin A, also called Peptidyl­prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis­ and trans­ proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV­1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity.
Catalog #145442
ApplicationsSuitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml
Immunocytochemistry: 8­25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No817815
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
PurityPurified by Protein A or G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-165 from human Cyclophilin A, expressed in E. coli (Accession# P62937).
SpecificityRecognizes human Cyclophilin A in direct ELISAs and Western blots. Recognizes mouse and rat Cyclophilin in Western blots. Species Crossreactivity: ~50% recombinant human (rh) Cyclophilin E, <15% rhCyclophilin F, and <5% rhCyclophilin A­like 4 is observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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