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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Plexin B2 is a 240kD member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa20­466) and three IPT repeats (aa803­1092). The ECD may be cleaved into two subunits, a 170kD alpha­chain (aa20­1164) and an 80kD TM beta­chain that remain noncovalently­linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa67­638. Over aa20­1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.
Catalog #	145572
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	537219
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Purity	Purified by Protein A or G affinity chromatography.
Immunogen	Recombinant protein corresponding to aa20-1160 from human Plexin B2, CHO-derived (Accession# O15031).
Specificity	Recognizes human Plexin B2 in direct ELISAs.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.