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You are here:Home » Antibodies » Abs to Proteasomes » Anti -PSMA1 (Proteasome Subunit alpha Type-1, 30kD Prosomal Protein, PROS-30, Macropain Subunit C2, Multicatalytic Endopeptidase Complex Subunit C2, Proteasome Component C2, Proteasome nu Chain, HC2, NU, PROS30, PSC2)

Anti -PSMA1 (Proteasome Subunit alpha Type-1, 30kD Prosomal Protein, PROS-30, Macropain
Subunit C2, Multicatalytic Endopeptidase Complex Subunit C2, Proteasome Component C2,
Proteasome nu Chain, HC2, NU, PROS30, PSC2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
PSMA1 (Proteosome subunit alpha type-1; also 30kD prosomal protein/PROS30, HC2, proteasome component C2/PSC2, PSMA-1/alpha6, and NU) is a 30kD member of the peptidase T1A family of enzymes. It is widely expressed, and found in both cytoplasm and nucleus. Short-lived intracellular molecules (typically proteins) are enzymatically degraded by the 26S proteosome. This is a multisubunit 3D complex that is over 2000kD in size, and recognizes previously ubiquitinated proteins. The middle of this 26S complex is shaped like a barrel with four staves that run circumferentially rather than longitudinally. Each stave contains seven subunits, with beta-type subunits generating the two center staves, and alpha-type subunits comprising the outer, or flanking staves. The function of the barrel, also known as the 20S protease core "particle", is to enzymatically cleave substrates that enter its chamber. For proteins, this is done by beta-type subunits. The 26S complex also cleaves mRNA, and this is mediated by alpha-type subunits. PSMA-1/alpha6 does not cleave mRNA, but it does positively regulate PSMA5/alpha5 catalytic activity. Notably, PSMA1 has also been reported to bind to LPS. Human PSMA1 is 263 amino acids (aa) in length. It contains an acetylated Met at position #1, plus three utilized phosphorylation sites at Tyr6, Thr11, and Ser16. There are at least four potential isoform variants. Three utilize alternative start sites. One shows a start site at Met140, while a second and third initiates translation at sites 6 and 43aa upstream of the standard site, respectively. A fourth isoform possess a 16aa substitution for aa115-263. Over aa16-263, human PSMA1 shares 98% aa sequence identity with mouse PSMA1.
Catalog #145585
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.2ug/ml
IOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No785019
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
PurityPurified by Protein A or G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa16-263 from human PSMA1, expressed in E. coli (Accession# P25786).
SpecificityRecognizes human PSMA1 in direct ELISAs. Recognizes endogenous mouse and rat PSMA1 in Western blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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