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You are here:Home » Antibodies » Abs to Protein Kinases, Ribosomal » Anti -RPS6 (Ribosomal Protein 56, Phosphoprotein NP33)

Anti -RPS6 (Ribosomal Protein 56, Phosphoprotein NP33)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
(40S) Ribosomal protein S6 (rpS6; also Phosphoprotein NP33) is a 32­34kD member of the ribsosmal protein S6e family of molecules. It is ubiquitously expressed and involved in the regulation of cell size. Higher eukaryotic ribosomes contain a small (or 40S) and large (or 60S) subunit. The small subunits are composed of one RNA molecule plus more than 30 individual proteins, one of which is rp6S. It is a substrate of both PASKIN and S6 kinase, and when phosphorylated C­terminally, appears to slow the rate of protein synthesis. Notably, the activities of rpS6 are complex, as phosphorylation seems to decrease cell proliferation and glucose utilization on one hand, while increasing cell size on the other. Human ribosomal protein S6 is 249 amino acids (aa) in length. It possesses two acetylated lysines plus six phosphorylated serines. There are three potential splice forms. One contains a four aa substitution for aa1­46, a second utilizes an alternative start site at Met32, and a third utilizes the same Met32 start site coupled to a substitution of a Gln residue for aa234­243. Full­length human, mouse and rat rpS6 are identical in their amino acid sequences.
Catalog #145598
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-249 from human Ribosomal Protein 56, expressed in E. coli (Accession# P62753).
SpecificityRecognizes human, mouse and rat Ribosomal Protein S6 in Western blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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