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You are here:Home » Antibodies » Antibodies-Sprouty » Anti -SPRED3, ID (Spred-3, Sprouty-related, EVH1 Domain-containing Protein 3, EVE-3, Spred-3)

Anti -SPRED3, ID (Spred-3, Sprouty-related, EVH1 Domain-containing Protein 3, EVE-3,


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist. This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.
Catalog #146326
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml
Immunofluorescence: 20ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Brain Tissue Lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 16aa from near the internal region of human Spred3.
SpecificityRecognizes human Spred3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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