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You are here:Home » Molecular Biology » Growth Factors-TGF » TGFB1, Recombinant, Human (Transforming Growth Factor beta-1, TGF-beta-1, TGFB)

TGFB1, Recombinant, Human (Transforming Growth Factor beta-1, TGF-beta-1, TGFB)

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Specifications

The three mammalian isoforms of TGF-b, TGF-b1, b2, b3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-b isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-b1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-b1 is a 25kD protein with each subunit containing 112aa, linked by a single disulfide bond.
Catalog #149585
SourceRecombinant protein corresponding to human TGF beta 1, expressed in A293 cells.
Biological ActivityDetermined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 was found to be 0.05ng/ml.
Specific Activity2x10e7units/mg
Endotoxin<0.1ng/ug (1EU/ug)
AA SequenceALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceA293 cells
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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