Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » LTA, Recombinant, Human (Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor Necrosis Factor Ligand Superfamily Member 1, TNFB, TNFSF1)

LTA, Recombinant, Human (Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor Necrosis Factor
Ligand Superfamily Member 1, TNFB, TNFSF1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF superfamily of ligands and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes incuding cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells. Recombinant human TNF-beta is a secreted 172aa protein,18.6kD, which forms an active non-disulfide linked homotrimer structure in solution. Due to glycosylation, TNF-beta has an approximate MW of 19.3kD based on SDS-PAGE gel and Mass Spectrometry.
Catalog #149639
SourceRecombinant protein corresponding to human TNF-beta, expressed in (BTI-Tn-5B1-4) High-5 Insect Cells.
Biological ActivityDetermined by the cytolysis of murine L929 cells in the presence of Actinomycin D. The ED50 was found to be 0.05ng/ml.
Specific Activity2x10e7units/mg
Endotoxin<0.1ng/ug (1EU/ug)
AA SequenceGVGLTPSAAQ TARQHPKMHL AHSTLKPAAH LIGDPSKQNS LLWRANTDRA FLQDGFSLSN NSLLVPTSGI YFVYSQVVFS GKAYSPKATS SPLYLAHEVQ LFSSQYPFHV PLLSSQKMVY PGLQEPWLHS MYHGAAFQLT QGDQLSTHTD GIPHLVLSPS TVFFGAFAL
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source(BTI-Tn-5B1-4) High-5 Insect Cells
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links