Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor AA, Recombinant, Mouse (PDGF-AA, Platelet-Derived Growth Factor Subunit A, PDGF Subunit A, Platelet-Derived Growth Factor A Chain, Platelet-Derived Growth Factor alpha Polypeptide, PDGF-1, PDGFA, PDGF1)

Platelet Derived Growth Factor AA, Recombinant, Mouse (PDGF-AA, Platelet-Derived Growth
Factor Subunit A, PDGF Subunit A, Platelet-Derived Growth Factor A Chain, Platelet-Derived
Growth Factor alpha Polypeptide, PDGF-1, PDGFA, PDGF1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

PDGFs are disulfide-linked dimers consisting of two 12-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-b. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-b interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-AA is a 28.7kD disulfide-linked homodimer of two A chains, 250aa.
Catalog #149710
SourceRecombinant protein corresponding to mouse PDGF-AA, expressed in E.coli.
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10ng/ml.
Endotoxin<0.1ng/ug (1EU/ug)
AA SequenceMSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links