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You are here:Home » Molecular Biology » Growth Factors-GDNF » Persephin, Recombinant, Mouse (PSP, Pspn)

Persephin, Recombinant, Mouse (PSP, Pspn)

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Specifications

Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR? (alpha1-alpha4) receptors. The GFR 4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. Persephin promotes the survival of ventral midbrain dompaminergic neurons and motor neurons after sciatic nerve oxotomy, and like GNDF, promotes ureteric bud branching. However, in contrast to GDNF and Neurturin, Persephin does not support survival of peripheral neurons. Recombinant murine Persephin is a disulfide-linked homodimer, composed of two 10.3kD polypeptide chains (96aa). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Catalog #149734
SourceRecombinant protein corresponding to mouse Persephin, expressed in E.coli.
Biological ActivityDetermined by its ability to stimulate proliferation of human thyroid carcinoma cells (TT cells). The ED50 was found to be <0.1ng/ml.
Specific Activity>1x10e7units/mg
Endotoxin<0.1ng/ug (1EU/ug)
AA SequenceALAGSCRLWS LTLPVAELGL GYASEEKVIF RYCAGSCPQE ARTQHSLVLA RLRGRGRAHG RPCCQPTSYA DVTFLDDQHH WQQLPQLSAA ACGCGG
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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