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You are here:Home » Growth Factors, Cytokines » Growth Factors-GDNF » Gdnf, Recombinant, Mouse (Glial Cell Line-derived Neurotrophic Factor, mGDNF, Astrocyte-derived Trophic Factor, ATF, AI385739)

Gdnf, Recombinant, Mouse (Glial Cell Line-derived Neurotrophic Factor, mGDNF,
Astrocyte-derived Trophic Factor, ATF, AI385739)

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Specifications

GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFR a (a1-a4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson's disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kD polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Catalog #149737
SourceRecombinant protein corresponding to mouse GDNF, expressed in E.coli.
Biological ActivityDetermined by the proliferation of rat C6 cells. The ED50 was found to be 0.2ng/ml.
Specific Activity5x10e6units/mg
Endotoxin<0.1ng/ug (1EU/ug)
AA SequenceMSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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