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You are here:Home » Molecular Biology » Growth Factors-Other » NRG1, Recombinant, Human (GGF, HGL, HRGA, NDF, SMDF, Pro-neuregulin-1, Membrane-bound Isoform, Neuregulin-1, Acetylcholine Receptor-inducing Activity, Breast Cancer Cell Differentiation Factor p45, Glial Growth Factor, Heregulin, Neu Differentiation Facto

NRG1, Recombinant, Human (GGF, HGL, HRGA, NDF, SMDF, Pro-neuregulin-1, Membrane-bound
Isoform, Neuregulin-1, Acetylcholine Receptor-inducing Activity, Breast Cancer Cell
Differentiation Factor p45, Glial Growth Factor, Heregulin, Neu Differentiation Facto

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Specifications

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-B1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-B1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-B1 (HRG1-B1) is a 7.5kD polypeptide consisting of only the EGF domain of heregulin-B1 (65aa).
Catalog #149744
SourceRecombinant protein corresponding to human Heregulin-B1, expressed in E.coli.
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of human MCF-7 cells. The ED50 was found to be 0.5ng/ml
Specific Activity2x10e6units/mg
Endotoxin<0.1ng/ug (1EU/ug)
AA SequenceSHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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